منابع مشابه
Studies on T34nduced Ribonucleic Acid Polvmerase
A procedure for the isolation and purification of bacteriophage T34nduced RNA polymerase from T34nfected Escherichia coli cells is presented. The procedure described leads to the isolation of polymerase preparation that displays a single protein band by polyacrylamide gel electrophoresis and is free of detectable ribonuclease and deoxyribonuclease activities. Other enzyme activities absent from...
متن کاملStudies on Liver Messenger Ribonucleic Acid.
Strong evidence has accumulated in recent years in favor of the following scheme of protein biosynthesis. DNA directs the formation of complementary strands of messenger RNA which possess the necessary genetic information to determine the sequential arrangement of amino acids in polypeptide chains (1, 2). The messenger RNA attaches itself to ribosomes to form a multiple ribosomal structure, the...
متن کاملPhysical and kinetic studies of arginyl transfer ribonucleic acid ligase of Neurospora. A sequential ordered mechanism.
The arginyl-tRNA ligase of Neurosporo has been purified 1,800-fold. The purified preparation catalyzed the esterification of 140 /Imoles of arginine to tRNA per hour per mg of protein at 35”. Polyacrylamide gel electrophoresis revealed a single major band from which the enzyme was estimated to be 95% homogeneous. The apparent molecular weight of the enzyme is about 85,000 as determined by sodiu...
متن کاملStudies on the Solubilized Ribonucleic Acid Polymerase from Rat
1. By using ultrasonic treatment in media of high ionic strength, the RNA polymerase activities associated with prostatic nuclei and nucleoli can be completely solubilized. Such enzyme preparations are entirely dependent on the provision of added DNA for full activity. 2. The solubilized enzymes from the nucleolar and extranucleolar regions can be separated by ion-exchange chromatography. 3. Ba...
متن کاملStudies on Termination of in Vitro Ribonucleic Acid Synthesis
The mechanism of action of the protein rho (p) in DNAdependent RNA synthesis has been examined. While p can interact with DNA to form a protein-DNA complex, detailed studies of this interaction suggest that this is not the mode of action of p. p appears to interact with Escherichia coli RNA polymerase at specific DNA sites leading to the formation of RNA chains of well defined length. RNA chain...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)81259-0